Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus.

ABSTRACT: The urine of bank voles (Myodes glareolus) contains substantial quantities of a small protein that is expressed at much higher levels in males than females, and at higher levels in males in the breeding season. This protein was purified and completely sequenced at the protein level by mass spectrometry. Leucine/isoleucine ambiguity was completely resolved by metabolic labelling, monitoring the incorporation of dietary deuterated leucine into specific sites in the protein. The predicted mass of the sequenced protein was exactly consonant with the mass of the protein measured in bank vole urine samples, correcting for the formation of two disulfide bonds. The sequence of the protein revealed that it was a lipocalin related to aphrodisin and other odorant-binding proteins (OBPs), but d

SUBMITTER: Loxley GM 

PROVIDER: S-EPMC5627053 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature