Multiple Mechanisms Drive Calcium Signal Dynamics around Laser-Induced Epithelial Wounds.

ABSTRACT: Epithelial wound healing is an evolutionarily conserved process that requires coordination across a field of cells. Studies in many organisms have shown that cytosolic calcium levels rise within a field of cells around the wound and spread to neighboring cells, within seconds of wounding. Although calcium is a known potent second messenger and master regulator of wound-healing programs, it is unknown what initiates the rise of cytosolic calcium across the wound field. Here we use laser ablation, a commonly used technique for the precision removal of cells or subcellular components, as a tool to investigate mechanisms of calcium entry upon wounding. Despite its precise ablation capabilities, we find that this technique damages cells outside the primary wound via a laser-induced cavitation b

SUBMITTER: Shannon EK 

PROVIDER: S-EPMC5627067 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature