Molecular trade-offs in RNA ligases affected the modular emergence of complex ribozymes at the origin of life.

ABSTRACT: In the RNA world hypothesis complex, self-replicating ribozymes were essential. For the emergence of an RNA world, less is known about the early processes that accounted for the formation of complex, long catalysts from small passively formed molecules. The functional role of small sequences has not been fully explored and, here, a possible role for smaller ligases is demonstrated. An established RNA polymerase model, the R18, was truncated from the 3' end to generate smaller molecules. All the molecules were investigated for self-ligation functions with a set of oligonucleotide substrates without predesigned base pairing. The smallest molecule that exhibited self-ligation activity was a 40-nucleotide RNA. It also demonstrated the greatest functional flexibility as it was more general in t

SUBMITTER: Dhar N 

PROVIDER: S-EPMC5627087 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature