Sensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector.

ABSTRACT: MicroRNAs (miRNAs) are small noncoding RNAs that modulate gene expression at the post-transcriptional level. Different types of cells express unique sets of miRNAs that can be exploited as potential molecular markers to identify specific cell types. Among the variety of miRNA detection methods, a fluorescence-based imaging system that utilises a fluorescent-reporter gene regulated by a target miRNA offers a major advantage for long-term tracking of the miRNA in living cells. In this study, we developed a novel fluorescence-based miRNA-monitoring system using a non-integrating cytoplasmic RNA vector based on a replication-defective and persistent Sendai virus (SeVdp). Because SeVdp vectors robustly and stably express transgenes, this system enabled sensitive monitoring of miRNAs by fluoresc

SUBMITTER: Sano M 

PROVIDER: S-EPMC5627244 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature