Single-cell screening of multiple biophysical properties in leukemia diagnosis from peripheral blood by pure light scattering.

ABSTRACT: Histology and histopathology are based on the morphometric observations of quiescent cells. Their diagnostic potential could largely benefit from a simultaneous screening of intrinsic biophysical properties at single-cell level. For such a purpose, we analyzed light scattering signatures of individual mononuclear blood cells in microfluidic flow. In particular, we extracted a set of biophysical properties including morphometric (dimension, shape and nucleus-to-cytosol ratio) and optical (optical density) ones to clearly discriminate different cell types and stages. By considering distinctive ranges of biophysical properties along with the obtained relative cell frequencies, we can identify unique cell classes corresponding to specific clinical conditions (p < 0.01). Based on such a straigh

SUBMITTER: Dannhauser D 

PROVIDER: S-EPMC5627307 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature