Species and genotype diversity of Plasmodium in malaria patients from Gabon analysed by next generation sequencing.

ABSTRACT:

Background

Six Plasmodium species are known to naturally infect humans. Mixed species infections occur regularly but morphological discrimination by microscopy is difficult and multiplicity of infection (MOI) can only be evaluated by molecular methods. This study investigated the complexity of Plasmodium infections in patients treated for microscopically detected non-falciparum or mixed species malaria in Gabon.

Methods

Ultra-deep sequencing of nucleus (18S rRNA), mitochondrion, and apicoplast encoded genes was used to evaluate Plasmodium species diversity and MOI in 46 symptomatic Gabonese patients with microscopically diagnosed non-falciparum or mixed species malaria.

Results

Deep sequencing revealed a large complexity of confections in patients with uncomplicated m

SUBMITTER: Lalremruata A 

PROVIDER: S-EPMC5627438 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature