Berkeley Screen: a set of 96 solutions for general macromolecular crystallization.

ABSTRACT: Using statistical analysis of the Biological Macromolecular Crystallization Database, combined with previous knowledge about crystallization reagents, a crystallization screen called the Berkeley Screen has been created. Correlating crystallization conditions and high-resolution protein structures, it is possible to better understand the influence that a particular solution has on protein crystal formation. Ions and small molecules such as buffers and precipitants used in crystallization experiments were identified in electron density maps, highlighting the role of these chemicals in protein crystal packing. The Berkeley Screen has been extensively used to crystallize target proteins from the Joint BioEnergy Institute and the Collaborative Crystallography program at the Berkeley Center for

SUBMITTER: Pereira JH 

PROVIDER: S-EPMC5627680 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature