Heterogeneous Electrochemical Aptamer-Based Sensor Surfaces for Controlled Sensor Response.

ABSTRACT: Structure-switching sensors utilize recognition elements that undergo a conformation change upon target binding that is converted into a quantitative signal. Electrochemical, aptamer-based sensors achieve detection of analytes through a conformation change in an electrode-bound, oligonucleotide aptamer by measuring changes in electron transfer efficiencies. The analytical performance of these sensors is related to the magnitude of the conformation change of the aptamer. The goal of the present work is to develop a general method to predictably tune the analytical performance (sensitivity and linear range) of electrochemical, aptamer-based sensors by utilizing a mixture of rationally designed aptamer sequences that are specific for the same target but with different affinities on the same e

SUBMITTER: Schoukroun-Barnes LR 

PROVIDER: S-EPMC5627764 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature