Renal cold storage followed by transplantation impairs expression of key mitochondrial fission and fusion proteins.

ABSTRACT:

Background

The majority of transplanted kidneys are procured from deceased donors which all require exposure to cold storage (CS) for successful transplantation. Unfortunately, this CS leads to renal and mitochondrial damage but, specific mitochondrial targets affected by CS remain largely unknown. The goal of this study is to determine whether pathways involved with mitochondrial fusion or fission, are disrupted during renal CS.

Methods

Male Lewis rat kidneys were exposed to cold storage (CS) alone or cold storage combined with transplantation (CS/Tx). To compare effects induced by CS, kidney transplantation without CS exposure (autotransplantation; ATx) was also used. Mitochondrial function was assessed using high resolution respirometry. Expression of mitochondrial fusion

SUBMITTER: Parajuli N 

PROVIDER: S-EPMC5627902 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature