Abnormal regulation of extracellular matrix and adhesion molecules in corneas of patients with keratoconus.

ABSTRACT:

Aim

To identify changes in the expression of genes coding for extracellular matrix (ECM) proteins in patients with non-inflammatory corneal disorder keratoconus (KC), patients with corneal scarring, and normal controls.

Materials and methods

Total RNA extracted from corneal tissue of 13 KC patients, 2 patients with corneal scaring and 4 normal controls was analyzed using Human Extracellular Matrix & Adhesion Molecules Profiler PCR Array. Statistically significant changes in gene expression were identified using the Data Analysis software.

Results

Comparison of KC and control corneas with thresholds of 1.5 or greater fold change and a p-value of 0.05 or lower, revealed 21 differentially expressed genes, 16 genes were downregulated and 5 were upregulated. Among transcri

SUBMITTER: Bykhovskaya Y 

PROVIDER: S-EPMC5627975 | biostudies-literature | 2016 May-Aug

REPOSITORIES: biostudies-literature