Improved conversion of ginsenoside Rb₁ to compound K by semi-rational design of Sulfolobus solfataricus β-glycosidase.

ABSTRACT: Ginsenoside compound K has been used as a key nutritional and cosmetic component because of its anti-fatigue and skin anti-aging effects. β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is known as the most efficient enzyme for compound K production. The hydrolytic pathway from ginsenoside Rb₁ to compound K via Rd and F₂ is the most important because Rb₁ is the most abundant component in ginseng extract. However, the enzymatic conversion of ginsenoside Rd to F₂ is a limiting step in the hydrolytic pathway because of the relatively low activity for Rd. A V209 residue obtained from error-prone PCR was related to Rd-hydrolyzing activity, and a docking pose showing an interaction with Val209 was selected from numerous docking poses. W361F was obt

SUBMITTER: Shin KC 

PROVIDER: S-EPMC5628084 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature