Immune Escape in Breast Cancer During In Situ to Invasive Carcinoma Transition.

ABSTRACT: To investigate immune escape during breast tumor progression, we analyzed the composition of leukocytes in normal breast tissues, ductal carcinoma in situ (DCIS), and invasive ductal carcinomas (IDC). We found significant tissue and tumor subtype-specific differences in multiple cell types including T cells and neutrophils. Gene expression profiling of CD45⁺CD3⁺ T cells demonstrated a decrease in CD8⁺ signatures in IDCs. Immunofluorescence analysis showed fewer activated GZMB⁺CD8⁺ T cells in IDC than in DCIS, including in matched DCIS and recurrent IDC. T-cell receptor clonotype diversity was significantly higher in DCIS than in IDCs. Immune checkpoint protein TIGIT-expressing T cells were more frequent in DCIS, whereas high PD-

SUBMITTER: Gil Del Alcazar CR 

PROVIDER: S-EPMC5628128 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature