Isolation and characterization of a novel endo-β-1,4-glucanase from a metagenomic library of the black-goat rumen.

ABSTRACT: The various types of lignocellulosic biomass found in plants comprise the most abundant renewable bioresources on Earth. In this study, the ruminal microbial ecosystem of black goats was explored because of their strong ability to digest lignocellulosic forage. A metagenomic fosmid library containing 115,200 clones was prepared from the black-goat rumen and screened for a novel cellulolytic enzyme. The KG35 gene, containing a novel glycosyl hydrolase family 5 cellulase domain, was isolated and functionally characterized. The novel glycosyl hydrolase family 5 cellulase gene is composed of a 963-bp open reading frame encoding a protein of 320 amino acid residues (35.1kDa). The deduced amino acid sequence showed the highest sequence identity (58%) for sequences from the glycosyl hydrolase fam

SUBMITTER: Song YH 

PROVIDER: S-EPMC5628298 | biostudies-literature | 2017 Oct - Dec

REPOSITORIES: biostudies-literature