Global analysis of ginsenoside Rg1 protective effects in β-amyloid-treated neuronal cells.

ABSTRACT:

Background

A number of reports have described the protective effects of ginsenoside Rg1 (Rg1) in Alzheimer's disease (AD). However, the protective mechanisms of Rg1 in AD remain elusive.

Methods

To investigate the potential mechanisms of Rg1 in β-amyloid peptide-treated SH-SY5Y cells, a comparative proteomic analysis was performed using stable isotope labeling with amino acids in cell culture combined with nano-LC-MS/MS.

Results

We identified a total of 1,149 proteins in three independent experiments. Forty-nine proteins were significantly altered by Rg1 after exposure of the cells to β-amyloid peptides. The protein interaction network analysis showed that these altered proteins were clustered in ribosomal proteins, mitochondria, the actin cytoskeleton, and splicing p

SUBMITTER: Shim JS 

PROVIDER: S-EPMC5628347 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature