Synergistic oligodeoxynucleotide strongly promotes CpG-induced interleukin-6 production.

ABSTRACT:

Background

Bacterial genomes span a significant portion of diversity, reflecting their adaptation strategies; these strategies include nucleotide usage biases that affect chromosome configuration. Here, we explore an immuno-synergistic oligodeoxynucleotide (iSN-ODN, named iSN34), derived from Lactobacillus rhamnosus GG (LGG) genomic sequences, that exhibits a synergistic effect on immune response to CpG-induced immune activation.

Methods

The sequence of iSN34 was designed based on the genomic sequences of LGG. Pathogen-free mice were purchased from Japan SLC and maintained under temperature- and light-controlled conditions. We tested the effects of iSN34 exposure in vitro and in vivo by assessing effects on mRNA expression, protein levels, and cell type in murine splenocytes

SUBMITTER: Nigar S 

PROVIDER: S-EPMC5628431 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature