Myocardial Reparative Properties of Cardiac Mesenchymal Cells Isolated on the Basis of Adherence.

ABSTRACT:

Background

The authors previously reported that the c-kit-positive (c-kit^POS) cells isolated from slowly adhering (SA) but not from rapidly adhering (RA) fractions of cardiac mesenchymal cells (CMCs) are effective in preserving left ventricular (LV) function after myocardial infarction (MI).

Objectives

This study evaluated whether adherence to plastic alone, without c-kit sorting, was sufficient to isolate reparative CMCs.

Methods

RA and SA CMCs were isolated from mouse hearts, expanded in vitro, characterized, and evaluated for therapeutic efficacy in mice subjected to MI.

Results

Morphological and phenotypic analysis revealed that murine RA and SA CMCs are indistinguishable; nevertheless, transcriptome analysis showed that they possess fundamentall

SUBMITTER: Wysoczynski M 

PROVIDER: S-EPMC5628504 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature