Intradermal Immunization with rAAV1 Vector Induces Robust Memory CD8⁺ T Cell Responses Independently of Transgene Expression in DCs.

ABSTRACT: Recombinant adeno-associated viral (rAAV) vectors exhibit interesting properties as vaccine carriers for their ability to induce long-lasting antibody responses. However, rAAV-based vaccines have been suggested to trigger functionally impaired long-term memory CD8⁺ T cell responses, in part due to poor dendritic cell (DC) transduction. Such results, albeit limited to intramuscular immunization, undermined the use of rAAV as vaccine vehicles against intracellular pathogens. We report here that intradermal immunization with a model rAAV2/1-based vaccine drives the development of bona fide long-term memory CD8⁺ T cell responses. The intradermal route of immunization and the presence of potent major histocompatibility complex (MHC) class II responses showed synergistic ef

SUBMITTER: Ghenassia A 

PROVIDER: S-EPMC5628776 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature