Design and validation of a 90K SNP genotyping assay for the water buffalo (Bubalus bubalis).

ABSTRACT:

Background

The availability of the bovine genome sequence and SNP panels has improved various genomic analyses, from exploring genetic diversity to aiding genetic selection. However, few of the SNP on the bovine chips are polymorphic in buffalo, therefore a panel of single nucleotide DNA markers exclusive for buffalo was necessary for molecular genetic analyses and to develop genomic selection approaches for water buffalo. The creation of a 90K SNP panel for river buffalo and testing in a genome wide association study for milk production is described here.

Methods

The genomes of 73 buffaloes of 4 different breeds were sequenced and aligned against the bovine genome, which facilitated the identification of 22 million of sequence variants among the buffalo genomes. Based on fr

SUBMITTER: Iamartino D 

PROVIDER: S-EPMC5628821 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature