Frequency-selective electrokinetic enrichment of biomolecules in physiological media based on electrical double-layer polarization.

ABSTRACT: Proteomic biomarkers of interest to the early diagnosis of diseases and infections are present at trace levels versus interfering species. Hence, their selective enrichment is needed within bio-assays for speeding binding kinetics with receptors and for reducing signal interferences. While DC fields can separate biomolecules based on their electrokinetic mobilities, they are unable to selectively enrich biomarkers versus interfering species, which may possess like-charges. We present the utilization of AC electrokinetics to enable frequency-selective enrichment of nanocolloidal biomolecules, based on the characteristic time constant for polarization of their electrical double-layer, since surface conduction in their ion cloud depends on colloidal size, shape and surface charge. In this man

SUBMITTER: Rohani A 

PROVIDER: S-EPMC5629073 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature