Adherence potential of indigenous lactic acid bacterial isolates obtained from fermented foods of Western Himalayas to intestinal epithelial Caco-2 and HT-29 cell lines.

ABSTRACT: The adherence of bacteria to epithelial cells and mucosal surfaces is a prerequisite for their colonization in the gut and a key criterion for the selection of probiotics. In this study, the eleven indigenous lactic acid bacterial isolates obtained from traditional fermented foods of Western Himalayas were screened for their adherence potential to intestinal epithelial cell lines. The level of adherence of eleven indigenous isolates to Caco-2 and HT-29 cell lines varied from 2.45 ± 0.5 to 9.55 ± 0.76% and 4.11 ± 0.68 to 12.88 ± 0.63%, respectively. Percent adhesion of indigenous isolates to Caco-2 cells was relatively lower as compared to HT-29 cells. Indigenous isolate AdF10 (L. plantarum) was found to be the most adhesive to HT-29 and Caco-2 with corresponding figures of 12.88 ± 0.63 and

SUBMITTER: Sharma S 

PROVIDER: S-EPMC5629159 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature