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Making good use of the byproducts of cultivation: green synthesis and antibacterial effects of silver nanoparticles using the leaf extract of blueberry.

ABSTRACT: In this study, we made an effort to use blueberry leaves extract as a reducing and a capping agent to biosynthesize silver nanoparticles (AgNPs). The successful formation of AgNPs was confirmed with UV-visible spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. Furthermore, their antimicrobial activities against four kinds of multi drug resistant clinical pathogens and aquatic pathogens were investigated. The results showed that as-prepared AgNPs exhibited broad-spectrum antibacterial activities. The antibacterial effects of the AgNPs based on the structural damage and DNA degradation of the tested bacteria were also studied. The green synthesis of AgNPs using blueberry leaf extract, which provided a new idea on the use of the byproducts of blueberry harvesting.

SUBMITTER: Li K

PROVIDER: S-EPMC5629166 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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Making good use of the byproducts of cultivation: green synthesis and antibacterial effects of silver nanoparticles using the leaf extract of blueberry.

Li Kun K Ma Chenyu C Jian Tiancai T Sun Hushan H Wang Lei L Xu Hui H Li Weihuan W Su Hongyan H Cheng Xianhao X

Journal of food science and technology 20170829 11

In this study, we made an effort to use blueberry leaves extract as a reducing and a capping agent to biosynthesize silver nanoparticles (AgNPs). The successful formation of AgNPs was confirmed with UV-visible spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. Furthermore, their antimicrobial activities against four kinds of multi drug resistant clinical pathogens and aquatic pathogens were investigated. The results showed that as-prepa ...[more]

PMID: 29051652

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Project description:A simple and rapid microwave assisted method of green synthesis of silver nanoparticles (AgNPs) was developed using aqueous leaf extract of Eucalyptus globulus(ELE), and their antibacterial and antibiofilm potential investigated. With this aim, the aqueous solutions of ELE and AgNO3(1 mM) were mixed (1:4 v/v), and microwave irradiated at 2450 Mhz, for 30 sec. The instant color change of the ELE-AgNO3 mixture from pale yellow to dark brown indicated ELE-AgNPs synthesis. The intensity of peak at 428 nm in UV-Vis spectra, due to the surface plasmon resonance of AgNPs, varied with the amount of ELE, AgNO3 concentration, pH and time of incubation. The biosynthesized ELE-AgNPs were characterized by UV-visible spectroscopy, XRD, TEM, SEM-EDX, FTIR and TGA analyses. The size of ELE-AgNPs was determined to be in range of 1.9-4.3 nm and 5-25 nm, with and without microwave treatment, respectively. SEM exhibited the capping of AgNPs with the ELE constituents, and validated by FTIR analysis. The FTIR data revealed the presence of plant organic constituents and metabolites bound to ELE-AgNPs, which contributes for their stability. The antimicrobial activity of ELE-AgNPs was assessed by growth and biofilm inhibition of extended spectrum β-lactamase (ESBL) producing Pseudomonas aeruginosa, Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) clinical bacterial isolates. The results demonstrated that S. aureus were more sensitive to ELE-AgNPs than E. coli and P. aeruginosa. MRSA exhibited higher sensitive than MSSA, whereas P. aeruginosa were more sensitive than E. coli to ELE-AgNPs treatment. Also, significant (83 ± 3% and 84 ± 5%) biofilm inhibition was observed in case of S. aureus and P. aeruginosa, respectively. The results elucidated environmentally friendly, economical and quick method for production of colloidal bio-functionalized ELE-AgNPs, for effectual clinical applications, as broad spectrum antibacterial agents and biofilm inhibitors.

Project description:So far, several studies have focused on the synthesis of metallic nanoparticles making use of extracts from the fruit of the plants from the genus Capsicum. However, as the fruit is the edible, and highly commercial, part of the plant, in this work we focused on the leaves, a part of the plant that is considered agro-industrial waste. The biological synthesis of gold (AuNPs) and silver (AgNPs) nanoparticles using aqueous extracts of root, stem and leaf of Capsicum chinense was evaluated, obtaining the best results with the leaf extract. Gold and silver nanoparticles synthesized using leaf extract (AuNPs-leaf and AgNPs-leaf, respectively) were characterized by UV-visible spectrophotometry (UV-Vis), Fourier Transform Infrared Spectroscopy with Attenuated Total Reflection (FTIR-ATR), X-ray Photoelectron Spectroscopy (XPS), Ultra Hight Resolution Scanning Electron Microscopy coupled to Energy-Dispersive X-ray spectroscopy (UHR-SEM-EDX) and Transmission Electron Microscopy (TEM), and tested for their antioxidant and antimicrobial activities. In addition, different metabolites involved in the synthesis of nanoparticles were analyzed. We found that by the use of extracts derived from the leaf, we could generate stable and easy to synthesize AuNPs and AgNPs. The AuNPs-leaf were synthesized using microwave radiation, while the AgNPs-leaf were synthesized using UV light radiation. The antioxidant activity of the extract, determined by ABTS, showed a decrease of 44.7% and 60.7% after the synthesis of the AuNPs-leaf and AgNPs-leaf, respectively. After the AgNPs-leaf synthesis, the concentration of polyphenols, reducing sugars and amino acids decreased by 15.4%, 38.7% and 46.8% in the leaf extract, respectively, while after the AuNPs-leaf synthesis only reducing sugars decreased by 67.7%. These results suggest that these groups of molecules are implicated in the reduction/stabilization of the nanoparticles. Although the contribution of these compounds in the synthesis of the AuNPs-leaf and the AgNPs-leaf was different. Finally, the AgNPs-leaf inhibited the growth of S. aureus, E. coli, S. marcescens and E. faecalis. All of them are bacterial strains of clinical importance due to their fast antibiotic resistance development.

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Dataset Information

Making good use of the byproducts of cultivation: green synthesis and antibacterial effects of silver nanoparticles using the leaf extract of blueberry.

Publications

Making good use of the byproducts of cultivation: green synthesis and antibacterial effects of silver nanoparticles using the leaf extract of blueberry.

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