Genetic engineering of the Calvin cycle toward enhanced photosynthetic CO₂ fixation in microalgae.

ABSTRACT:

Background

Photosynthetic microalgae are emerging as potential biomass feedstock for sustainable production of biofuels and value-added bioproducts. CO₂ biomitigation through these organisms is considered as an eco-friendly and promising alternative to the existing carbon sequestration methods. Nonetheless, the inherent relatively low photosynthetic capacity of microalgae has hampered the practical use of this strategy for CO₂ biomitigation applications.

Results

Here, we demonstrate the feasibility of improving photosynthetic capacity by the genetic manipulation of the Calvin cycle in the typical green microalga Chlorella vulgaris. Firstly, we fused a plastid transit peptide to upstream of the enhanced green fluorescent protein (EGFP) and confirm

SUBMITTER: Yang B 

PROVIDER: S-EPMC5629779 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature