FUT8 promotes breast cancer cell invasiveness by remodeling TGF-β receptor core fucosylation.

ABSTRACT:

Background

Core fucosylation (addition of fucose in α-1,6-linkage to core N-acetylglucosamine of N-glycans) catalyzed by fucosyltransferase 8 (FUT8) is critical for signaling receptors involved in many physiological and pathological processes such as cell growth, adhesion, and tumor metastasis. Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) regulates the invasion and metastasis of breast tumors. However, whether receptor core fucosylation affects TGF-β signaling during breast cancer progression remains largely unknown.

Method

In this study, gene expression profiling and western blot were used to validate the EMT-associated expression of FUT8. Lentivirus-mediated gain-of-function study, short hairpin RNA (shRNA) or CRISPR/Cas9-mediated lo

SUBMITTER: Tu CF 

PROVIDER: S-EPMC5629780 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature