Lysine methylation of FEN1 by SET7 is essential for its cellular response to replicative stress.

ABSTRACT: The DNA damage response (DDR) is central to the cell survival and it requires post-translational modifications, in part, to sense the damage, amplify the signaling response and recruit and regulate DNA repair enzymes. Lysine methylation of histones such as H4K20 and non-histone proteins including p53 has been shown to be essential for the mounting of the DDR. It is well-known that the lysine methyltransferase SET7 regulates the DDR, as cells lacking this enzyme are hypersensitive to chemotherapeutic drugs. To define addition substrates of SET7 involved in the DDR, we screened a peptide array encompassing potential lysine methylation sites from >100 key DDR proteins and identified peptides from 58 proteins to be lysine methylated defining a methylation consensus sequence of [S>K^-2

SUBMITTER: Thandapani P 

PROVIDER: S-EPMC5630301 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature