Generation and evaluation of antibody agents for molecular imaging of CD44v6-expressing cancers.

ABSTRACT:

Aim

The aim of this study was to generate and characterize scFv antibodies directed to human CD44v6, as well as to radiolabel and evaluate top candidates in vitro and in vivo for their potential use in CD44v6-targeted molecular imaging in cancer patients.

Materials and methods

Phage display selections were used to isolate CD44v6-specific scFvs. A chain shuffling strategy was employed for affinity maturation based on a set of CD44v6-specific first-generation clones. Two second-generation scFv clones were then chosen for labeling with ¹¹¹In or ¹²⁵I and assessed for CD44v6-specific binding on cultured tumor cells. In vivo uptake and distribution was evaluated in tumor-bearing mice using a dual tumor model. Finally, a proof-of-concept s

SUBMITTER: Haylock AK 

PROVIDER: S-EPMC5630320 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature