The deubiquitinating enzyme USP5 promotes pancreatic cancer via modulating cell cycle regulators.

ABSTRACT: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal solid tumors. With an overall five-year survival rate remaining below 6%, there is an explicit need to search for new molecular targets for therapeutic interventions. We undertook a barcode labelled short-hairpin (shRNA) library screen in pancreatic cancer cells in order to identify novel genes promoting cancer survival and progression. Among the candidate genes identified in this screen was the deubiquitinase USP5, which subsequent gene expression analyses demonstrated to be significantly upregulated in primary human pancreatic cancer tissues. Using different knockdown approaches, we show that expression of USP5 is essential for the proliferation and survival of pancreatic cancer cells, tested under different

SUBMITTER: Kaistha BP 

PROVIDER: S-EPMC5630405 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature