Deconstructing a multiple antibiotic resistance regulation through the quantification of its input function.

ABSTRACT: Many essential bacterial responses present complex transcriptional regulation of gene expression. To what extent can the study of these responses substantiate the logic of their regulation? Here, we show how the input function of the genes constituting the response, i.e., the information of how their transcription rates change as function of the signals acting on the regulators, can serve as a quantitative tool to deconstruct the corresponding regulatory logic. To demonstrate this approach, we consider the multiple antibiotic resistance (mar) response in Escherichia coli. By characterizing the input function of its representative genes in wild-type and mutant bacteria, we recognize a dual autoregulation motif as main determinant of the response, which is further adjusted by t

SUBMITTER: Rodrigo G 

PROVIDER: S-EPMC5630622 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature