Quantitation of putative colorectal cancer biomarker candidates in serum extracellular vesicles by targeted proteomics.

ABSTRACT: At the moment, there is no sensitive clinical test for detecting early-stage colorectal cancer (CRC). Target proteomics has enabled high-throughput verification of hundreds of biomarker candidate proteins. Using this technology, we verified 725 previously reported CRC biomarker candidate proteins that are functionally correlated with CRC in extracellular vesicles (EVs) from patients. Of these, 356 proteins were quantified, and 34 peptides (22 proteins) showed significant differences in the serum EVs between healthy controls and CRC patients of two independent cohorts (n = 77 and 84). These peptides were evaluated as single or multiple markers, and four single peptides in annexin family proteins and eight combinations of peptides showed area under the curve > 0.9 for discriminating between

SUBMITTER: Shiromizu T 

PROVIDER: S-EPMC5630664 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature