Mettl3-/Mettl14-mediated mRNA N⁶-methyladenosine modulates murine spermatogenesis.

ABSTRACT: Spermatogenesis is a differentiation process during which diploid spermatogonial stem cells (SSCs) produce haploid spermatozoa. This highly specialized process is precisely controlled at the transcriptional, posttranscriptional, and translational levels. Here we report that N⁶-methyladenosine (m⁶A), an epitranscriptomic mark regulating gene expression, plays essential roles during spermatogenesis. We present comprehensive m⁶A mRNA methylomes of mouse spermatogenic cells from five developmental stages: undifferentiated spermatogonia, type A₁ spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes, and round spermatids. Germ cell-specific inactivation of the m⁶A RNA methyltransferase Mettl3 or Mettl14 with Vasa-Cre c

SUBMITTER: Lin Z 

PROVIDER: S-EPMC5630681 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature