Interrogation of side chain biases for oligomannose recognition by antibody 2G12 via structure-guided phage display libraries.

ABSTRACT: Monoclonal antibodies (mAbs) are essential reagents for deciphering gene or protein function and have been a fruitful source of therapeutic and diagnostic agents. However, developing anticarbohydrate antibodies to target glycans for those purposes has been less successful because the molecular basis for glycan-mAb interactions is poorly understood relative to protein- or peptide-binding mAbs. Here, we report our investigation on glycan-mAb interactions by using the unique architectural scaffold of 2G12, an antibody that targets oligomannoses on the HIV-1 glycoprotein gp120, as the template for engineering highly specific mAbs to target glycans. We first analyzed 24 different X-ray structures of antiglycan mAbs from the Protein Data Bank to determine side chain amino acid distributions in o

SUBMITTER: Lin TY 

PROVIDER: S-EPMC5632219 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature