Complicated Staphylococcus aureus Bacteremia (SAB) Is Associated with Genetic Variation in GLS2

ABSTRACT: Abstract

Background

SAB is a serious, common infection. We used whole exome sequencing (WES) to examine the cumulative effect of coding variants in each gene on risk of complicated SAB in a discovery set of patients, and then evaluated the nominally significant genes in a replication set of patients using custom-capture sequencing.

Methods

The discovery set comprised 84 complicated SAB cases (endocarditis or bone/joint infection) frequency-matched by age (in deciles), sex, and bacterial clonal complex (CC5/30, CC8) to 84 uncomplicated SAB controls. All were white inpatients at Duke University. WES utilized Agilent SureSelect 72Mb capture kits, followed by sequencing on an Illumina HiSeq2000, alignment and base calling with a standard pipeline. The SKAT-O and EPACTS packag

SUBMITTER: Fowler V 

PROVIDER: S-EPMC5632279 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature