Development and validation of a multi-locus DNA metabarcoding method to identify endangered species in complex samples.

ABSTRACT: DNA metabarcoding provides great potential for species identification in complex samples such as food supplements and traditional medicines. Such a method would aid Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) enforcement officers to combat wildlife crime by preventing illegal trade of endangered plant and animal species. The objective of this research was to develop a multi-locus DNA metabarcoding method for forensic wildlife species identification and to evaluate the applicability and reproducibility of this approach across different laboratories. A DNA metabarcoding method was developed that makes use of 12 DNA barcode markers that have demonstrated universal applicability across a wide range of plant and animal taxa and that facilitate the ide

SUBMITTER: Arulandhu AJ 

PROVIDER: S-EPMC5632295 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature