Inhibitory effect of saliva on osteoclastogenesis in vitro requires toll-like receptor 4 signaling.

ABSTRACT:

Objectives

Saliva can suppress osteoclastogenesis, but the underlying mechanism has not been discovered yet. Considering that endotoxins suppress osteoclastogenesis in bone marrow cultures and that saliva contains endotoxins, it was reasonable to hypothesize that the impact of saliva on osteoclastogenesis requires toll-like receptor 4 signaling.

Material and methods

To test this hypothesis, we blocked toll-like receptor 4 signaling with TAK-242 in the presence of saliva in murine bone marrow cultures. Osteoclastogenesis was evaluated based on gene expression analysis and histochemical staining for tartrate-resistant acid phosphatase. Resorption was performed on dentine.

Results

We report that TAK-242 reversed the inhibitory effect of fresh sterile saliva on the format

SUBMITTER: Muller HD 

PROVIDER: S-EPMC5632348 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature