Highly Efficient Single-Step Enrichment of Low Abundance Phosphopeptides from Plant Membrane Preparations.

ABSTRACT: Mass spectrometry (MS)-based large scale phosphoproteomics has facilitated the investigation of plant phosphorylation dynamics on a system-wide scale. However, generating large scale data sets for membrane phosphoproteins usually requires fractionation of samples and extended hands-on laboratory time. To overcome these limitations, we developed "ShortPhos," an efficient and simple phosphoproteomics protocol optimized for research on plant membrane proteins. The optimized workflow allows fast and efficient identification and quantification of phosphopeptides, even from small amounts of starting plant materials. "ShortPhos" can produce label-free datasets with a high quantitative reproducibility. In addition, the "ShortPhos" protocol recovered more phosphorylation sites from membrane protein

SUBMITTER: Wu XN 

PROVIDER: S-EPMC5632542 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature