Influence of Standard Laboratory Procedures on Measures of Erythrocyte Damage.

ABSTRACT: The ability to characterize the mechanical properties of erythrocytes is important in clinical and research contexts: to diagnose and monitor hematologic disorders, as well as to optimize the design of cardiovascular implants and blood circulating devices with respect to blood damage. However, investigation of red blood cell (RBC) properties generally involves preparatory and processing steps. Even though these impose mechanical stresses on cells, little is known about their impact on the final measurement results. In this study, we investigated the effect of centrifuging, vortexing, pipetting, and high pressures on several markers of mechanical blood damage and RBC membrane properties. Using human venous blood, we analyzed erythrocyte damage by measuring free hemoglobin, phosphatidylserin

SUBMITTER: Wiegmann L 

PROVIDER: S-EPMC5632557 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature