ImmunoPET imaging of tissue factor expression in pancreatic cancer with ⁸⁹Zr-Df-ALT-836.

ABSTRACT: Overexpression of tissue factor (TF) has been associated with increased tumor growth, tumor angiogenesis, and metastatic potential in many malignancies, including pancreatic cancer. Additionally, high TF expression was shown to strongly correlate with poor prognoses and decreased survival in pancreatic cancer patients. Herein, we exploited the potential targeting of TF for positron emission tomography (PET) imaging of pancreatic cancer. The TF-targeted tracer was developed through radiolabeling of the anti-human TF monoclonal antibody (ALT-836) with ⁸⁹Zr. The tracer was characterized by fluorescence microscopy and flow cytometry assays in BXPC-3 and PANC-1 cells, two pancreatic cancer cell lines with high and low TF expression levels, respectively. Non-invasive PET scans were ac

SUBMITTER: Hernandez R 

PROVIDER: S-EPMC5632593 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature