Fate Decision Between Group 3 Innate Lymphoid and Conventional NK Cell Lineages by Notch Signaling in Human Circulating Hematopoietic Progenitors.

ABSTRACT: The role of Notch signaling in human innate lymphoid cell (ILC) differentiation is unclear, although IL-7 and IL-15 promote differentiation of natural cytotoxicity receptor (NCR) NKp44⁺ group 3 ILCs (NCR⁺ILC3s) and conventional NK (cNK) cells from CD34⁺ hematopoietic progenitor cells (HPCs) ex vivo. In this study, we analyzed the functions of Notch in the differentiation of NCR⁺ILC3s and cNK cells from human HPC subpopulations circulating in peripheral blood by limiting dilution and clonal assays using high-throughput flow cytometry. We demonstrated that Notch signaling in combination with IL-7 induced NCR⁺ILC3 differentiation, but conversely suppressed IL-15-dependent cNK cell generation in CD45RA⁺Flt-3^-c-Kit

SUBMITTER: Kyoizumi S 

PROVIDER: S-EPMC5632839 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature