Preservation of the metaproteome: variability of protein preservation in ancient dental calculus.

ABSTRACT: Proteomic analysis of dental calculus is emerging as a powerful tool for disease and dietary characterisation of archaeological populations. To better understand the variability in protein results from dental calculus, we analysed 21 samples from three Roman-period populations to compare: 1) the quantity of extracted protein; 2) the number of mass spectral queries; and 3) the number of peptide spectral matches and protein identifications. We found little correlation between the quantity of calculus analysed and total protein identifications, as well as no systematic trends between site location and protein preservation. We identified a wide range of individual variability, which may be associated with the mechanisms of calculus formation and/or post-depositional contamination, in addition

SUBMITTER: Mackie M 

PROVIDER: S-EPMC5633013 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature