Secretory pathway Ca²⁺ -ATPases promote in vitro microcalcifications in breast cancer cells.

ABSTRACT: Calcification of the breast is often an outward manifestation of underlying molecular changes that drive carcinogenesis. Up to 50% of all non-palpable breast tumors and 90% of ductal carcinoma in situ present with radiographically dense mineralization in mammographic scans. However, surprisingly little is known about the molecular pathways that lead to microcalcifications in the breast. Here, we report on a rapid and quantitative in vitro assay to monitor microcalcifications in breast cancer cell lines, including MCF7, MDA-MB-231, and Hs578T. We show that the Secretory Pathway Ca²⁺ -ATPases SPCA1 and SPCA2 are strongly induced under osteogenic conditions that elicit microcalcifications. SPCA gene expression is significantly elevated in breast cancer subtypes that are associated

SUBMITTER: Dang D 

PROVIDER: S-EPMC5633510 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature