Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes.

ABSTRACT: Lymphocytes are immune cells that are critical for the maintenance of adaptive immunity. Differentiation of lymphoid progenitors yields B-, T-, and NK-cell subtypes that individually correlate with specific forms of leukemia or lymphoma. Therefore, it is imperative a precise method of cell categorization is utilized to detect differences in distinct disease states present in patients. One viable means of classification involves evaluation of the cell surface proteome of lymphoid malignancies. Specifically, this manuscript details the use of an antibody independent approach known as Cell Surface Capture Technology, to assess the N-glycoproteome of four human lymphocyte cell lines. Altogether, 404 cell surface N-glycoproteins were identified as markers for specific cell types involved in lym

SUBMITTER: Haverland NA 

PROVIDER: S-EPMC5633517 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature