Defining the current scope and limitations of dual noncanonical amino acid mutagenesis in mammalian cells.

ABSTRACT: The ability to site-specifically incorporate two distinct noncanonical amino acids (ncAAs) into the proteome of a mammalian cell with high fidelity and efficiency will have many enabling applications. It would require the use of two different engineered aminoacyl-tRNA synthetase (aaRS)/tRNA pairs, each suppressing a distinct nonsense codon, and which cross-react neither with each other, nor with their counterparts from the host cell. Three different aaRS/tRNA pairs have been developed so far to expand the genetic code of mammalian cells, which can be potentially combined in three unique ways to drive site-specific incorporation of two distinct ncAAs. To explore the suitability of using these combinations for suppressing two distinct nonsense codons with high fidelity and efficiency, here w

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC5633785 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature