Proton beam irradiation inhibits the migration of melanoma cells.

ABSTRACT:

Purpose

In recent years experimental data have indicated that low-energy proton beam radiation might induce a difference in cellular migration in comparison to photons. We therefore set out to compare the effect of proton beam irradiation and X-rays on the survival and long-term migratory properties of two cell lines: uveal melanoma Mel270 and skin melanoma BLM.

Materials and methods

Cells treated with either proton beam or X-rays were analyzed for their survival using clonogenic assay and MTT test. Long-term migratory properties were assessed with time-lapse monitoring of individual cell movements, wound test and transpore migration, while the expression of the related proteins was measured with western blot.

Results

Exposure to proton beam and X-rays led to similar

SUBMITTER: Jasinska-Konior K 

PROVIDER: S-EPMC5634624 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature