Dye diffusion at surfaces: charge matters.

ABSTRACT: Fluorescence correlation spectroscopy and single molecule burst analysis were used to measure the effects of glass surface interactions on the diffusion of two common fluorescent dyes, one cationic and one anionic. The effects of dye-surface interactions on measured diffusion rates as a function of distance from the surface were investigated. Use of a three-axis piezo stage, combined with reference calibration measurements, enabled the accurate acquisition of surface-distance-dependent transport data. This analysis reveals attractive interactions between the cationic dye and the surface, which significantly alter the extracted diffusion values and persist in the measurements up to 1.0 microm from the surface. The Coulomb attraction between the cationic dye and the surface also results in r

SUBMITTER: Daniels CR 

PROVIDER: S-EPMC5634710 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature