Comparative Metabolomics of Mycoplasma bovis and Mycoplasma gallisepticum Reveals Fundamental Differences in Active Metabolic Pathways and Suggests Novel Gene Annotations.

ABSTRACT: Mycoplasmas are simple, but successful parasites that have the smallest genome of any free-living cell and are thought to have a highly streamlined cellular metabolism. Here, we have undertaken a detailed metabolomic analysis of two species, Mycoplasma bovis and Mycoplasma gallisepticum, which cause economically important diseases in cattle and poultry, respectively. Untargeted gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry analyses of mycoplasma metabolite extracts revealed significant differences in the steady-state levels of many metabolites in central carbon metabolism, while ¹³C stable isotope labeling studies revealed marked differences in carbon source utilization. These data were mapped onto in silico metabolic netwo

SUBMITTER: Masukagami Y 

PROVIDER: S-EPMC5634790 | biostudies-literature | 2017 Sep-Oct

REPOSITORIES: biostudies-literature