Contextual tumor suppressor function of T cell death-associated gene 8 (TDAG8) in hematological malignancies.

ABSTRACT:

Background

Extracellular acidosis is a condition found within the tumor microenvironment due to inadequate blood perfusion, hypoxia, and altered tumor cell metabolism. Acidosis has pleiotropic effects on malignant progression; therefore it is essential to understand how acidosis exerts its diverse effects. TDAG8 is a proton-sensing G-protein-coupled receptor that can be activated by extracellular acidosis.

Methods

TDAG8 gene expression was analyzed by bioinformatic analyses and quantitative RT-PCR in human hematological malignancies. Retroviral transduction was used to restore TDAG8 expression in U937, Ramos and other blood cancer cells. Multiple in vitro and in vivo tumorigenesis and metastasis assays were employed to evaluate the effects of TDAG8 expression on blood cancer

SUBMITTER: Justus CR 

PROVIDER: S-EPMC5634876 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature