The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading.

ABSTRACT:

Background

The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNA-silencing pathways It exerts a pivotal role in microRNA maturation and activity and can modulate chromatin remodeling, transcriptional gene regulation and RNA splicing. Estrogen receptor beta (ERβ) is endowed with oncosuppressive activities, antagonizing hormone-induced carcinogenesis and inhibiting growth and oncogenic functions in luminal-like breast cancers (BCs), where its expression correlates with a better prognosis of the disease.

Results

Applying interaction proteomics coupled to mass spectrometry to characterize nuclear factors cooperating with ERβ in gene regulation, we identify AGO2 as a novel partner of ERβ in human BC cells. ERβ-AGO2 association was confirmed in vitro and in vivo in b

SUBMITTER: Tarallo R 

PROVIDER: S-EPMC5634881 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature