Optimization of culture conditions for rapid clinical-scale expansion of human umbilical cord blood-derived mesenchymal stem cells.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) have broad-spectrum therapeutic effects in various diseases, and thus have many clinical applications. However, it is difficult to produce sufficient numbers of MSCs for clinical use, and improved culture systems are required. Here, we report the effects of calcium (Ca²⁺) and hypoxia on the proliferation of human umbilical cord blood-derived MSCs (hUCB-MSCs). In addition, we determined the optimal conditions of these two factors for the large-scale culture of hUCB-MSCs.

Methods

hUCB-MSCs were maintained under hypoxic conditions (3% O₂) with 1.8 mM Ca²⁺ during long-term culture, and their proliferation was evaluated. To characterize the underlying mechanisms, the effects on hypoxia-inducible factor (HIF

SUBMITTER: Choi W 

PROVIDER: S-EPMC5634990 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature