Phosphine inhibits transcription of the catalase gene through the DRE/DREF system in Drosophila melanogaster.

ABSTRACT: Phosphine (PH₃) is a toxin commonly used for pest control. Its toxicity is attributed primarily to its ability to induce oxidative damage. Our previous work showed that phosphine could disrupt the cell antioxidant defence system by inhibiting expression of the catalase gene in Drosophila melanogaster (DmCAT). However, the exact mechanism of this inhibition remains unclear. Here, we implemented a luciferase reporter assay driven by the DmCAT promoter in D. melanogaster S2 cells and showed that this reporter could be inhibited by phosphine treatment. A minimal fragment of the promoter (-94 to 0 bp), which contained a DNA replication-related element (DRE) consensus motif (-78 to -85 bp), was sufficient for phosphine-mediated reporter inhibition, suggesting the involvement of the tr

SUBMITTER: Liu T 

PROVIDER: S-EPMC5635064 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature