The Small Rho GTPases Rac1 and Rac2 Are Important for T-Cell Independent Antigen Responses and for Suppressing Switching to IgG2b in Mice.

ABSTRACT: The Rho GTPases Cdc42, Rac1, and Rac2 coordinate receptor signaling to cell adhesion, migration, and proliferation. Deletion of Rac1 and Rac2 early during B cell development leads to failure in B cell entry into the splenic white pulp. Here, we sought to understand the role of Rac1 and Rac2 in B cell functionality and during the humoral antibody response. To circumvent the migratory deficiency of B cells lacking both Rac1 and Rac2, we took the approach to inducibly delete Rac1 in Rac2^-/- B cells in the spleen (Rac1^BRac2^-/- B cells). Rac1^BRac2^-/- mice had normal differentiation of splenic B cell populations, except for a reduction in marginal zone B cells. Rac1^BRac2^-/- B cells showed normal spreading response on an

SUBMITTER: Gerasimcik N 

PROVIDER: S-EPMC5635268 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature