Increased lipid production by heterologous expression of AtWRI1 transcription factor in Nannochloropsis salina.

ABSTRACT:

Background

Genetic engineering of microalgae is necessary to produce economically feasible strains for biofuel production. Current efforts are focused on the manipulation of individual metabolic genes, but the outcomes are not sufficiently stable and/or efficient for large-scale production of biofuels and other materials. Transcription factors (TFs) are emerging as good alternatives for engineering of microalgae, not only to increase production of biomaterials but to enhance stress tolerance. Here, we investigated an AP2 type TF Wrinkled1 in Arabidopsis (AtWRI1) known as a key regulator of lipid biosynthesis in plants, and applied it to industrial microalgae, Nannochloropsis salina.

Results

We expressed AtWRI1 TF heterologously in N. salina, named

SUBMITTER: Kang NK 

PROVIDER: S-EPMC5635583 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature